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  • Title: Fatty acid analysis of triacylglycerols: Preparation of fatty acid methyl esters for gas chromatography.
    Author: Ichihara K, Kohsaka C, Tomari N, Kiyono T, Wada J, Hirooka K, Yamamoto Y.
    Journal: Anal Biochem; 2016 Feb 15; 495():6-8. PubMed ID: 26656926.
    Abstract:
    A method to prepare fatty acid methyl esters was developed for fatty acid analysis of triacylglycerols by gas chromatography (GC). Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was converted to methyl oleate with an average yield of 99.3%. This procedure gave reliable and reproducible data on fatty acid compositions determined by GC.
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