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  • Title: Development and Validation of a PCR Assay To Detect the Prairie Epidemic Strain of Pseudomonas aeruginosa from Patients with Cystic Fibrosis.
    Author: Workentine M, Poonja A, Waddell B, Duong J, Storey DG, Gregson D, Somayaji R, Rabin HR, Surette MG, Parkins MD.
    Journal: J Clin Microbiol; 2016 Feb; 54(2):489-91. PubMed ID: 26659208.
    Abstract:
    The monitoring of epidemic Pseudomonas aeruginosa is important for cystic fibrosis (CF) infection control. The prairie epidemic strain (PES) is common in western Canadian CF clinics. Using whole-genome sequencing, we identified a novel genomic island and developed a PCR assay for PES. Against a collection of 186 P. aeruginosa isolates, the assay had 98% sensitivity and 100% specificity.
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