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  • Title: Identification and molecular characterization of Panax notoginseng virus A, which may represent an undescribed novel species of the genus Totivirus, family Totiviridae.
    Author: Guo L, Yang X, Wu W, Tan G, Fang S, Zhang S, Li F.
    Journal: Arch Virol; 2016 Mar; 161(3):731-4. PubMed ID: 26660166.
    Abstract:
    A double-stranded RNA (dsRNA), PN-S, isolated from Panax notoginseng was sequenced and characterized. dsRNA PN-S was determined to be 5003 base pairs (bp) in length and to have a genome organization similar to that of totiviruses. The two deduced proteins encoded by dsRNA PN-S have the highest amino acid (aa) sequence identity to CP and RNA-dependent RNA polymerase (RdRp) of black raspberry virus F (BrVF), a tentative member of the genus Totivirus, family Totiviridae, at 39% and 54%, respectively. In a phylogenetic tree based on an alignment of the RdRp amino acid sequences of dsRNA PN-S and selected totiviruses, dsRNA PN-S formed a monophyletic clade with dsRNA viruses belonging to the genus Totivirus. These data suggest that dsRNA PN-S may represent the genome of a member of a novel species of the genus Totivirus, family Totiviridae, for which we propose the name Panax notoginseng virus A (PnVA).
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