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  • Title: [The study of anticoagulants selection in platelet-rich plasma preparation].
    Author: Hua L, Lai G, Zhenjun L, Guie M.
    Journal: Zhonghua Zheng Xing Wai Ke Za Zhi; 2015 Jul; 31(4):295-300. PubMed ID: 26665933.
    Abstract:
    OBJECTIVE: To investigate the effect of the anticoagulants on PRP quality, so as to clarify the appropriate anticoagulant used in PRP production. METHODS: The microstructure change of platelets collected via heparin, citrate, acid citrate dextrose (ACD) and citrate-theophylline-adenosine-dipyridamole ( CTAD) was observed by TEM following time course. The extent of spontaneous activation of platelets in four groups was detected by measuring sP-selectin in plasma. The TGF-β1 release amount of activated PRP of four groups was measured. RESULTS: CTAD is superior to other anticoagulants in maintaining the integrity of platelet structures for a long time and preventing platelet spontaneous activation. ACD slightly surpassed heparin and citrate in above two aspects. ACD-PRP and CTAD-PRP released significantly more TGF-β1 compared with heparin and citrate. CONCLUSIONS: The PRP quality and biological effects were strongly associated with the type of Anticoagulants. ACD and CTAD are optimal anticoagulants in PRP production for they can maintain platelet viability at a high level.
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