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  • Title: Purification and identification of a neutral endopeptidase in Plasmodium falciparum schizonts and merozoites.
    Author: Grellier P, Picard I, Bernard F, Mayer R, Heidrich HG, Monsigny M, Schrével J.
    Journal: Parasitol Res; 1989; 75(6):455-60. PubMed ID: 2666980.
    Abstract:
    An endopeptidase specific to the Plasmodium falciparum erythrocytic schizont stage and to free merozoites was detected using the fluorogenic GlcA-Val-Leu-Gly-Lys(or Arg)-AEC substrate. The enzyme was purified by high performance liquid chromatography (HPLC); its optimal activity was around pH 7.5 and its isoelectric point was 4.4. The molecular weight of the enzyme was about 68,000, as demonstrated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions. The endopeptidase was strongly inhibited by thiol proteinase inhibitors, leupeptin, and antipain. The possible involvement of this neutral endopeptidase in the reinvasion process is discussed.
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