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  • Title: Inactivation of the Escherichia coli heat-labile enterotoxin by in vitro mutagenesis of the A-subunit gene.
    Author: Harford S, Dykes CW, Hobden AN, Read MJ, Halliday IJ.
    Journal: Eur J Biochem; 1989 Aug 01; 183(2):311-6. PubMed ID: 2667996.
    Abstract:
    In vitro mutagenesis of the LTA gene, encoding the A subunit of the Escherichia coli heat-labile enterotoxin, has been used to obtain A subunits deficient in enzymic activity. One inactive A-subunit mutant which contained two amino acid substitutions, was shown to associate with native B subunits to form a holotoxoid lacking toxin activity. A serine to phenylalanine mutation appears to be responsible for the loss of toxicity.
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