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  • Title: [Substrate specificity of the biotransformation enzymes in a Nocardia erythropolis culture].
    Author: Klimashina MM, Viktorovskiĭ IV, Mikhaĭlova NP, V'iunov KA.
    Journal: Izv Akad Nauk SSSR Biol; 1989; (3):429-34. PubMed ID: 2668369.
    Abstract:
    Substrate specificity of biotransformation enzymes of culture Nocardia erythropolis was studied. Products of transformation of cholesterol and three sterols of microbial origin: ergosterol, ergosta-5,7-dien-3 beta-ol and ergosta-7,22-dien-3 beta-ol was identified with a help of thin-layer chromatography, UV spectrophotometry and mass-spectrometry. It was established, that delta 22-bond in the side chains of sterols and delta 7-bond slows and delta 5-bond makes impossible cleavage of side chains of sterols.
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