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  • Title: Evaluation of time-resolved fluoroimmunoassay with Eu-labelled protein-A for serum progesterone.
    Author: Ius A, Ferrara L, Meroni G, Bacigalupo MA.
    Journal: J Steroid Biochem; 1989 Jul; 33(1):101-3. PubMed ID: 2668643.
    Abstract:
    Progesterone has been assayed in several serum samples by a time-resolved fluoroimmunoassay (TR-FIA). The solid phase was 11 alpha-hydroxyprogesterone hemisuccinate bound covalently to ovalbumin and adsorbed on wells of polystyrene. The assay was based on competitive reaction of solid phase-bound hormone and samples with specific antibody labelled in situ with protein-A prelabelled with europium. The bound Eu was dissociated from the solid phase by an enhancement solution and measured by time-resolved fluorometry. The coefficient of correlation between TR-FIA and RIA was 0.97.
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