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  • Title: [Optimization of physico-chemical parameters of reaction media for determining the activity of RNA-polymerases of mollicutes].
    Author: Skripal' IG, Babichev VV.
    Journal: Mikrobiol Zh (1978); 1989; 51(2):66-71. PubMed ID: 2668709.
    Abstract:
    RNA-polymerases of mollicutes differ considerably from certain species of bacteria in the temperature optimum of the activity manifestation. The activity of mollicute enzymes is considerably higher in the presence of manganese ions than in the presence of magnesium ions. They differ from Escherichia coli transcriptase in this character but are similar to the RNA-polymerase of lactic bacteria, hypothetic ancestors of acholeplasm. Sensitivity of RNA-polymerases to metals may be one of arguments explaining phylogeny of mycoplasms. It is established that for studying mollicute transcriptase the reacting mixture for examining the enzymic activity besides the major components should have the following parameters: pH 8.0; the MnCl concentration--8-10 mM; for form II of the A. laidlawii subsp. granulum 118 enzyme--4.5 mM; ammonium sulphate concentration--40 mM; for form II of st. 118-20 mM; the reaction should be conducted at the temperature of 27 degrees C for 30 min.
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