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Title: Reversible immobilization of laccase onto metal-ion-chelated magnetic microspheres for bisphenol A removal. Author: Lin J, Liu Y, Chen S, Le X, Zhou X, Zhao Z, Ou Y, Yang J. Journal: Int J Biol Macromol; 2016 Mar; 84():189-99. PubMed ID: 26691384. Abstract: Increasing attention has been given to nanobiocatalysis for commercial applications. In this study, laccase was reversibly immobilized onto Cu(ΙΙ)- and Mn(ΙΙ)-chelated magnetic microspheres and successfully applied to remove bisphenol A (BPA) from water. The results indicated that the loading of laccase onto the metal-ion-chelated magnetic microspheres was approximately 100mg/g. After five successive adsorption-desorption cycles, the laccase adsorption capacities did not change. In comparison with free laccase, the thermal and storage stabilities of immobilized laccase were significantly improved. Immobilized laccase exhibited a high removal efficiency for BPA under the combined actions of biodegradation and adsorption. Greater than 85% of BPA was removed under optimum conditions. The effects of various factors on the BPA removal efficiency of immobilized laccase were analysed. The results showed that metal-ion-chelated magnetic microspheres have great potential for industrial applications.[Abstract] [Full Text] [Related] [New Search]