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  • Title: Calcium regulates glutamate dehydrogenase and poly-γ-glutamic acid synthesis in Bacillus natto.
    Author: Meng Y, Dong G, Zhang C, Ren Y, Qu Y, Chen W.
    Journal: Biotechnol Lett; 2016 Apr; 38(4):673-9. PubMed ID: 26712367.
    Abstract:
    OBJECTIVE: To study the effect of Ca(2+) on glutamate dehydrogenase (GDH) and its role in poly-γ-glutamic acid (γ-PGA) synthesis in Bacillus natto HSF 1410. RESULTS: When the concentration of Ca(2+) varied from 0 to 0.1 g/l in the growth medium of B. natto HSF 1410, γ-PGA production increased from 6.8 to 9.7 g/l, while GDH specific activity and NH4Cl consumption improved from 183 to 295 U/mg and from 0.65 to 0.77 g/l, respectively. GDH with α-ketoglutarate as substrate primarily used NADPH as coenzyme with a K m of 0.08 mM. GDH was responsible for the synthesis of endogenous glutamate. The specific activity of GDH remained essentially unchanged in the presence of CaCl2 (0.05-0.2 g/l) in vitro. However, the specific activity of GDH and its expression was significantly increased by CaCl2 in vivo. Therefore, the regulation of GDH and PGA synthesis by Ca(2+) is an intracellular process. CONCLUSION: Calcium regulation may be an effective approach for producing γ-PGA on an industrial scale.
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