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  • Title: Processing and secretion of barley (1-3,1-4)-beta-glucanase in yeast.
    Author: Olsen O, Thomsen KK.
    Journal: Carlsberg Res Commun; 1989; 54(2):29-39. PubMed ID: 2673277.
    Abstract:
    DNA segments encoding signal peptides from mouse alpha-amylase, yeast acid phosphatase, and yeast invertase were fused in frame to a barley (1-3,1-4)-beta-glucanase cDNA gene and expressed in yeast cells under the control of the phosphoglycerate kinase gene promoter. Pure beta-glucanase is obtained by gel filtration of concentrated yeast cell supernatant. It was shown that the glucanase pre-protein was specifically processed and the mature protein efficiently secreted when the yeast invertase signal sequence directed secretion.
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