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  • Title: Expression of IL-1 and tumor necrosis factor by endothelial cells: role in stimulating fibroblast PGE2 synthesis.
    Author: Shanahan WR, Hancock WW, Korn JH.
    Journal: J Exp Pathol; 1989; 4(1):17-27. PubMed ID: 2674374.
    Abstract:
    We describe the elaboration by endothelial cells of activity that stimulates fibroblast PGE2 production. Culture supernates of human umbilical vein endothelial cells (ECSN) at concentrations of 2.5 to 25% stimulated human foreskin fibroblast PGE2 production 6 to 180-fold. Following molecular sieve chromatography, peak activity eluted with an Mr of 14-18,000. In a standard IL-1 assay, neither ECSN or 14-18,000 Mr fractions possessing PGE2 stimulatory activity were able to stimulate murine thymocyte proliferation in response to PHA. Immunoperoxidase staining of endothelial cells demonstrated intracellular IL-1; after addition of exogenous IL-1 endothelial cells also stained for tumor necrosis factor (TNF). IL-1 and TNF are known to be synergistic in stimulating fibroblast PGE2 synthesis. Thus, elaboration of TNF by endothelial cells may allow detection of IL-1 in fibroblast PGE2 assays when the concentration of IL-1 is inadequate to stimulate thymocyte proliferation. Interactions of cytokines elaborated by cells may play an important role in effects on target cells.
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