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  • Title: Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3.
    Author: Reitsma M, Bastiaan-Net S, Sforza S, van der Valk JP, van Gerth van Wijk R, Savelkoul HF, de Jong NW, Wichers HJ.
    Journal: J Agric Food Chem; 2016 Feb 10; 64(5):1191-201. PubMed ID: 26769082.
    Abstract:
    In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.
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