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  • Title: [Rapid determination of microbial sensitivity to antibiotics by using laser spectroscopy].
    Author: Lytkin MI, Poliak MS, Petrov NN, Nikitin VP, Fateeva LI, Fotiadi AE, Chepcheruk GS, Tulipov AN.
    Journal: Vestn Khir Im I I Grek; 1989 May; 142(5):26-8. PubMed ID: 2678707.
    Abstract:
    A method of express-determination of the susceptibility of microbes to antibiotics is presented based upon the registration by the method of laser spectroscopy of changes of optic properties of cultures at early terms of their incubation in liquid nutrient media with addition of antibiotics. The method was elaborated in 13 standard strains of Staphylococcus aureus and Escherichia coli with antibiotics of different classes, tested on the microflora of pus of patients with sepsis, verified by the method of serial dilutions of the antibiotic. The method makes it possible to obtain the minimum inhibiting concentration of antimicrobial preparations 1 h after the moment of obtaining the native material.
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