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  • Title: [UNG-dependent correction of molecular heteroduplexes of M13 phage DNA in Escherichia coli cells].
    Author: Golubovskaia VM, Aprelikova ON, Tomilin NV.
    Journal: Mol Gen Mikrobiol Virusol; 1989 Jul; (7):24-9. PubMed ID: 2682222.
    Abstract:
    Correction of heteroduplex DNA obtained by hybridization of uracil-containing single-stranded M13mp18 phage DNA and "mutant" synthetic oligonucleotide with deletion of cytosine in SalGI site was studied in ung+ and ung- E. coli strains. Uracil-containing DNA was prepared after growth of phage in an E. coli strain dut- ung-. The DNA was hybridized with "mutant" oligonucleotide then complementary DNA chain was synthesized by T4 DNA polymerase. Ung+ and ung- E. coli cells were transformed by DNA. In all experiments mutation frequency in ung+ was higher than in ung- cells (approximately 6-fold) and reached 11-50%. Absolute number of mutants was higher in ung+ cells. The results indicate that high level of mutagenesis depends on uracil repair system polarizing the correction of heteroduplex DNA.
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