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  • Title: Antiproliferative Evaluation and Apoptosis Induction in MCF- 7 Cells by Ziziphus spina christi Leaf Extracts.
    Author: Farmani F, Moein M, Amanzadeh A, Kandelous HM, Ehsanpour Z, Salimi M.
    Journal: Asian Pac J Cancer Prev; 2016; 17(1):315-21. PubMed ID: 26838231.
    Abstract:
    BACKGROUND: Herbal medicine has becoming a potential source of treatment for different types of cancer including breast cancer. It has been shown that plants from the family Rhamnaceae possess anticancer activity. OBJECTIVE: In this study, we determined the antiproliferative influence of Ziziphus spina christi- a species from this family- on the MCF-7 (human breast adenocarcinoma) cell line. MATERIALS AND METHODS: The cytotoxicity of the total extract, ethanol, ethanol-aqueous (1:1) as well as aqueous fractions of Ziziphus spina christi leaves was evaluated through MTT assay against MCF-7 cell line. Cell cycle inhibition and apoptosis induction were assessed by flowcytometry cycle RNase/PI analysis and Annexin V-FLUOS, respectively. Apoptosis was also analyzed by immunoblotting assay. RESULTS: Our results indicated that the ethanolic fraction had the lowest IC50 value (0.02 mg/ml), induced cell cycle arrest at the G1/S phase as well as apoptosis after a 48h of treatment. CONCLUSIONS: This is the first report on anticancer effect of Ziziphus spina christi ethanolic fraction on breast cancer cells, providing a scientific basis for its utility in traditional medicine. However, further in-depth studies are needed to confirm the precise mechanisms.
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