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  • Title: Isolation and molecular identification of Naegleria fowleri from Nile river, Egypt.
    Author: Al-Herrawy AZ, Gad MA.
    Journal: J Egypt Public Health Assoc; 2015 Dec; 90(4):161-5. PubMed ID: 26854897.
    Abstract:
    BACKGROUND: Members of the genus Naegleria are free-living amoebae distributed in various aquatic environments. Naegleria fowleri is the only species that can cause fatal primary amoebic meningoencephalitis in humans. MATERIALS AND METHODS: A total of 48 Nile water samples were collected from the water stream passing though Cairo. The samples were processed for the detection of Naegleria spp. using non-nutrient agar at 45°C. The isolates of Naegleria spp. were identified based on the morphologic criteria of trophozoite, flagellated and cyst stages. Molecular characterization of the isolates was performed using PCR. RESULTS: The obtained results showed that Naegleria spp. were found in 45.8% of Nile water samples by means of microscopic examination. Seasonally, the highest prevalence of Naegleria spp. was recorded in summer (66.7%). Moreover, the highest prevalence of N. fowleri was recorded in summer (25%). CONCLUSION: The occurrence of heat-tolerant Naegleria spp., especially N. fowleri, in Nile water should be considered as a potential health threat.
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