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  • Title: Singlet oxygen mediated photobinding of 8-methoxypsoralen to DNA and genotoxicity in E. coli.
    Author: Beijersbergen van Henegouwen GM, Wijn ET, Schoonderwoerd SA, de Vries H, Dall'Acqua F.
    Journal: Z Naturforsch C J Biosci; 1989; 44(9-10):819-23. PubMed ID: 2686671.
    Abstract:
    Although oxygen dependent photoreactions of 8-methoxypsoralen (8-MOP) are known, damage to DNA is mostly considered to proceed via photocycloaddition to this biomacromolecule. In this study the survival of colony forming ability of E. coli K12/343, which is deficient in DNA-repair capability, appeared to be lower in D2O than in H2O after exposure to the combination of 8-MOP and UV-A. Photobinding to bacterial DNA was approximately 40% higher in D2O than in H2O. However this last difference was found only when the bacteria were kept in the reaction medium for 1 h at 37 degrees C after the irradiation was stopped and not when they were plated out immediately afterwards. The results indicate that in this bacterial test system 1O2 mediated photobinding of 8-MOP to DNA contributes to the genotoxic effect observed.
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