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  • Title: An automated reticulocyte counting method: preliminary observations.
    Author: Tatsumi N, Tsuda I, Kojima K, Niri M, Setoguchi K.
    Journal: Med Lab Sci; 1989 Apr; 46(2):157-60. PubMed ID: 2687609.
    Abstract:
    We evaluated the counting of reticulocytes in the peripheral blood with a newly developed flow cytometer type of automated counter that performs a single test within 60 s. The volume of sample needed is 100 microliters and the cells are stained with auramine-O in the counter. The mean within-run reproducibility was 4.66% (CV, n = 50), and dilution of blood gave highly linear results with an r value of 0.996. Correlation was good between manual reticulocyte counts and those performed with the counter (r = 0.893). Samples with large numbers of leucocytes, erythrocytes, or platelets did not interfere with the automated reticulocyte counting, and provided accurate and precise data.
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