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  • Title: Efficient conversion of crude glycerol from various industrial wastes into single cell oil by yeast Yarrowia lipolytica.
    Author: Dobrowolski A, Mituła P, Rymowicz W, Mirończuk AM.
    Journal: Bioresour Technol; 2016 May; 207():237-43. PubMed ID: 26890799.
    Abstract:
    In this study, crude glycerol from various industries was used to produce lipids via wild type Yarrowia lipolytica A101. We tested samples without any prior purification from five different waste products; each contained various concentrations of glycerol (42-87%) as the sole carbon source. The best results for lipid production were obtained for medium containing glycerol from fat saponification. This reached 1.69gL(-1) (25% of total cell dry weight) with a biomass yield of 0.17gg(-1) in the flasks experiment. The batch cultivation in a bioreactor resulted in enhanced lipid production-it achieved 4.72gL(-1) with a biomass yield 0.21gg(-1). Moreover, the properly selected batch of crude glycerol provides a defined fatty acid composition. In summary, this paper shows that crude glycerol from soap production could be efficiently converted to single cell oil without any prior purification.
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