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  • Title: Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil.
    Author: Cezar RD, Lucena-Silva N, Filho AF, Borges Jde M, de Oliveira PR, Lúcio ÉC, Arruda-Lima M, Santana VL, Pinheiro Junior JW.
    Journal: BMC Vet Res; 2016 Feb 20; 12():31. PubMed ID: 26897524.
    Abstract:
    BACKGROUND: The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly associated with bovine tuberculosis (BTB) were also evaluated. RESULTS: Of the 802 samples analyzed, one milk (0.25%) and eight blood (2%) samples were positive for M. bovis in the qPCR and their identities were confirmed by sequencing. Animals positive for M. bovis were found in six (30%) of the 20 properties visited. None of the risk factors evaluated were statistically associated with BTB. CONCLUSIONS: M. bovis DNA was detected in one milk sample what may pose a risk to public health because raw milk is commonly consumed in Brazil.
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