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  • Title: Recognition of nucleotide sequences at the Escherichia coli galactose operon P1 promoter by RNA polymerase.
    Author: Chan B, Busby S.
    Journal: Gene; 1989 Dec 14; 84(2):227-36. PubMed ID: 2693211.
    Abstract:
    Specific nucleotide (nt) sequences in the -35 region are not essential for galP1 promoter activity, whereas nt sequences in the spacer region are needed for transcription initiation: a G:C base pair at nt -14 and sequences upstream from this position are necessary. In the absence of these sequences, transcription initiation is dependent on the insertion of oligodeoxyribonucleotides carrying -35 region consensus hexamer sequences. Additionally, for maximal promoter activity, specific sequences just upstream from nt -49 are required. Because galP1 carries no sequence resembling the -35 region consensus hexamer, we propose that recognition by RNA polymerase proceeds via an unusual mechanism involving contacts upstream from the -10 hexamer, distortion of the spacer region and a contact upstream from nt -49.
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