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  • Title: [Cloning the pyruvate decarboxylase gene of Zymomonas mobilis and its expression in Escherichia coli].
    Author: Zverlov VV, Bankovskiĭ VK, Churikova OV, Mogutov MA, Iur'ev MZ.
    Journal: Mol Gen Mikrobiol Virusol; 1989 Sep; (9):11-3. PubMed ID: 2693955.
    Abstract:
    The pyruvate decarboxylase gene of Zymomonas mobilis CP4 has been cloned in Escherichia coli strain TG1 cells on the pUC18 vector plasmid. Activity of the enzyme in the lysates of the obtained clones is about 30 units per 1 mg of protein. Neither the dependence of the pyruvate decarboxylase activity on the presence of IPTG or glucose in the cultivation medium nor the difference in activity of the enzyme for the clones harbouring the recombinant plasmids with the different orientation of the pyruvate decarboxylase gene to lac-promoter have been demonstrated in the presented work. These facts imply the transcription of pyruvate decarboxylase gene from its own promoter.
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