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  • Title: [Immunoenzyme analysis of adenovirus hexon. Determination of antibodies from hyperimmunized chickens].
    Author: Khil'ko SN, Kirasova MA, Piker SD, Osidze SD, Fomina NV, Burgasova MP, Tikhonenko TI.
    Journal: Mol Gen Mikrobiol Virusol; 1989 Sep; (9):43-8. PubMed ID: 2693958.
    Abstract:
    We have elaborated three systems of enzyme-linked immunosorbent assay (ELISA) for detection of chicken IgG antibodies specific for hexon antigens of three immunologically distinct adenovirus groups: those of mammalian adenoviruses (Mastadenovira), typical avian adenoviruses (Aviadenovira) and of egg-drop syndrome-76 (EDS-76) virus. In each system the antibodies against respective hexons were specifically detected. In mammalian adenovirus hexons the ELISA detects primarily the type-specific (epsilon) and genus-specific (alpha) antigenic determinants. The time course of anti-hexon antibodies content was followed during immunization. The level of anti-hexon antibodies in egg yolk reflects adequately their content in blood serum. The technique is suitable for serological diagnosis of chicken adenoviral infections as well as for characterization of egg-yolk antibodies obtained by preparative hyperimmunization of hens.
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