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  • Title: [Direct gas chromatography analysis of volatile fatty acids and lactic acids in microbiological diagnosis].
    Author: Socolowsky S, Höhne C, Sandow D.
    Journal: Z Gesamte Hyg; 1989 Oct; 35(10):596-8. PubMed ID: 2694646.
    Abstract:
    The gas chromatographic analysis of short-chain fatty acids is of great significance in the rapid diagnosis of anaerobic infections and bacterial meningitis. A new analytical method is presented detecting lactic acid without prior derivatisation in addition to volatile fatty acids in the aqueous phase directly. Lactic acid levels greater than 30 mg/dl are measured in cerebrospinal fluids of patients with acute bacterial meningitis. The sole detection of acetic acid in concentrations higher than 20 mg/dl indicates aerobes in clinical materials. A high correlation exists between the determination of n-butyric acid as well as iso-valeric acid and the occurrence of anaerobic bacteria.
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