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  • Title: Highly specific quantification of microRNA by coupling probe-rolling circle amplification and Förster resonance energy transfer.
    Author: Wu X, Zhu S, Huang P, Chen Y.
    Journal: Anal Biochem; 2016 Jun 01; 502():16-23. PubMed ID: 26973220.
    Abstract:
    MicroRNA (miRNA) plays vital roles in various biological processes. In general, sensitivity and specificity are the major parameters for the quantification of miRNA. In this study, padlock probe-rolling circle amplification and Förster resonance energy transfer (pRCA-FRET) were coupled for specific and quantitative detection of miRNA. pRCA-FRET showed superior specificity to differentiate single-base mismatch and excellent sensitivity with a detection limit of 103 aM. The current method has the potential to quantify low amounts of miRNA in the same family for studies on their biological functions.
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