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  • Title: Polymerase chain reaction for the detection of Mycobacterium leprae.
    Author: Hartskeerl RA, de Wit MY, Klatser PR.
    Journal: J Gen Microbiol; 1989 Sep; 135(9):2357-64. PubMed ID: 2697743.
    Abstract:
    A polymerase chain reaction (PCR) using heat-stable Taq polymerase is described for the specific detection of Mycobacterium leprae, the causative agent of leprosy. A set of primers was selected on the basis of the nucleotide sequence of a gene encoding the 36 kDa antigen of M. leprae. With this set of primers in the PCR, M. leprae could be detected specifically with a detection limit approximating one bacterium. This PCR appears to meet the criteria of specificity and sensitivity required for a useful tool in epidemiology and eventually for the control of leprosy.
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