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  • Title: Overexpression of the truncated version of ILV2 enhances glycerol production in Saccharomyces cerevisiae.
    Author: Murashchenko L, Abbas C, Dmytruk K, Sibirny A.
    Journal: Yeast; 2016 Aug; 33(8):463-9. PubMed ID: 26990811.
    Abstract:
    Acetolactate synthase is a mitochondrial enzyme that catalyses the conversion of two pyruvate molecules to an acetolactate molecule with release of carbon dioxide. The overexpression of the truncated version of the corresponding gene, ILV2, that codes for presumably cytosolic acetolactate synthase in the yeast Saccharomyces cerevisiae, led to a decrease in intracellular pyruvate concentration. This recombinant strain was also characterized by a four-fold increase in glycerol production, with a concomitant 1.8-fold reduction in ethanol production, when compared to that of the wild-type strain under anaerobic conditions in a glucose alcoholic fermentation. Copyright © 2016 John Wiley & Sons, Ltd.
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