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  • Title: Expression of hepatitis B virus large envelope protein in Escherichia coli and Saccharomyces cerevisiae.
    Author: Korec E, Korcová J, Palková Z, Vondrejs V, Korínek V, Reinis M, Bichko VV, Hlozánek I.
    Journal: Folia Biol (Praha); 1989; 35(5):315-27. PubMed ID: 2699291.
    Abstract:
    The gene coding for hepatitis B large envelope protein was cloned under the lac promoter in bacterial vector pUC-8 and under the ADH1 promoter in yeast expression shuttle vector pVT103-U, and expression of HBsAg in bacteria and yeast was determined. The strongest expression of large envelope protein was obtained after transformation of the protease-deficient yeast strain BJ1991. The recombinant large envelope protein did not form complex 22-nm particles and was not secreted into medium.
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