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  • Title: Mumia xiangluensis sp. nov., isolated from the rhizosphere of Peucedanum praeruptorum Dunn.
    Author: Zhou S, Jia F, Liu C, Fan J, Li J, Bai Y, Wang X, Xiang W.
    Journal: Antonie Van Leeuwenhoek; 2016 Jun; 109(6):827-32. PubMed ID: 27027520.
    Abstract:
    During an investigation of microbial diversity in medicinal herbs, a novel actinobacterium, strain NEAU-KD1(T) was isolated from the rhizosphere of Peucedanum praeruptorum Dunn collected from Xianglu Mountain in Heilongjiang Province, northeast China and characterized using a polyphasic approach. The organism was found to have the typical chemotaxonomic and morphological characteristics of the genus Mumia. Cells were observed to be non-spore-forming and irregular cocci. The cell wall was found to contain LL-diaminopimelic acid as the cell wall diamino acid. The whole-cell sugars were detected as galactose and rhamnose and the predominant menaquinone was identified as MK-9(H4). The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid and five unidentified phospholipids. The major cellular fatty acids were determined to be composed of C16:0, 10-methyl C18:0 and C18:1ω7c. The phylogenetic analysis based on 16S rRNA gene sequence also indicated that strain NEAU-KD1(T) belongs to the genus Mumia and with high sequence similarity to Mumia flava NBRC 109973(T) (97.6 % sequence similarity). The results of DNA-DNA hybridization and the phenotypic characteristics indicated that strain NEAU-KD1(T) could be distinguished from its close phylogenetic relative. Thus, strain NEAU-KD1(T) can be concluded to represent a novel species of the genus Mumia, for which the name Mumia xiangluensis sp. nov. is proposed. The type strain is NEAU-KD1(T) (=CGMCC 4.7305(T) = DSM 101040(T)).
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