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  • Title: Enhanced succinic acid production in Corynebacterium glutamicum with increasing the available NADH supply and glucose consumption rate by decreasing H(+)-ATPase activity.
    Author: Xu H, Zhou Z, Wang C, Chen Z, Cai H.
    Journal: Biotechnol Lett; 2016 Jul; 38(7):1181-6. PubMed ID: 27053082.
    Abstract:
    OBJECTIVES: To enhance succinic acid production in Corynebacterium glutamicum by increasing the supply of NADH and the rate of glucose consumption by decreasing H(+)-ATPase activity. RESULTS: A mutant of C. glutamicum NC-3-1 with decreased H(+)-ATPase activity was constructed. This increased the rate of glycolysis and the supply of NADH. Fermentation of C. glutamicum NC-3-1 gave 39 % higher succinic acid production (113 and 81 g/l), a 29 % higher succinic acid yield (0.94 and 0.73 g succinic acid/g glucose) and decreased by-products formation compared to that of C. glutamicum NC-3 in 5 l bioreactor. CONCLUSION: The point mutation in C. glutamicum NC-3-1 increased the rate of glycolysis and resulted in higher succinic acid production, higher succinic acid yield and significantly decreased formation of by-products.
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