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  • Title: [Up-regulations of Bax and caspase-3 and down-regulation of Bcl-2 after Xinfeng capsule treatment in adjuvant-induced arthritis rats].
    Author: Jiang H, Liu J, Gao J, Meng M, Qin X, Wang T.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2016 Apr; 32(4):457-61. PubMed ID: 27053609.
    Abstract:
    OBJECTIVE: To observe the effects of Xinfeng capsule (XFC) treatment on the expressions of Bcl-2, Bax and caspase-3 in the synovium tissues of the adjuvant-induced arthritis (AA) rats and explore the possible mechanisms. METHODS: Spague-Dawley (SD) rats were randomly divided into normal group, model group, XFC (1.5, 3.0, 6.0 g/kg) groups and 40 mg/kg tripterygium glycosides tablet (TGT) group, with 10 rats in each group. Arthritis rat was induced by intracutaneous innoculation of 0.1 mL Freund's complete adjuvant (FCA) in the left paw. XFC at different doses and TGT, diluted in water, were administrated via gavage, once a day from the 19th day to the 48th day after AA induction. The normal and model rats were orally administered the same volume of saline solution. Then, ankle-joint samples were taken to examine the degree of AA by HE staining. Bcl-2, Bax and caspase-3 mRNA expressions were determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Meanwhile, the protein expression levels of Bcl-2, Bax and caspase-3 were detected by immunohistochemistry and Western blotting. RESULTS: Compared with the model group, XFC treatment improved histopathological changes, such as synovial hyperplaisia, pannus formation, and so on. Furthermore, XFC (3.0 and 6.0 g/kg) groups not only obviously attenuated mRNA and protein expressions of Bcl-2, but also obviously up-regulated the expressions of Bax and caspase-3 both at protein and mRNA levels. CONCLUSION: XFC has a protective effect on AA rats, which might be partly associated with its regulatory role in decreasing the expression of Bcl-2 and promoting the expressions of Bax and caspase-3.
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