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  • Title: Improved TLC systems for rapid resolution of phenyl thiohydantoin amino acids.
    Author: Bhushan R, Mahesh VK, Mallikharjun PV.
    Journal: Biomed Chromatogr; 1989 Jan; 3(1):43-5. PubMed ID: 2706365.
    Abstract:
    Two new solvent systems, n-hexane + propionic acid (26:5, v/v) and chloroform + acetone (29:3, v/v), for the rapid resolution and identification of an 18-component mixture of phenylthiohydantoin amino acids are reported. Using these systems certain difficult combinations of phenylthiohydantoin amino acids are resolved. Two more solvent systems, viz chloroform + acetic acid (27:3, v/v) and chloroform + methanol (30:4, v/v), are developed to resolve phenylthiodantoin derivatives of aspartic and glutamic acids.
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