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  • Title: In vitro reconstitution of hnRNP particles.
    Author: Schenkel J, Kloetzel PM.
    Journal: FEBS Lett; 1989 Apr 10; 247(1):51-4. PubMed ID: 2707451.
    Abstract:
    The assembly of hnRNP-like particles was studied by in vitro reconstitution, UV-crosslinking and CsCl-equilibrium centrifugation. Using total nuclear protein and RNA extracts from HeLa cells for RNP reconstitution, RNP particles sedimenting with the same buoyant density of p = 1.4 g/cm3 as 'native' 40 S core hnRNPs were obtained. Under the stringent reconstitution conditions used, hnRNP complexes containing only the Cl-core hnRNP protein could be identified.
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