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  • Title: Liquid chromatographic determination of carminic acid in yogurt.
    Author: Jalón M, Peña MJ, Rivas JC.
    Journal: J Assoc Off Anal Chem; 1989; 72(2):231-4. PubMed ID: 2708269.
    Abstract:
    A reverse-phase liquid chromatographic method is described for the determination of carminic acid in yogurt. A C18 column is used with acetonitrile-1.19M formic acid (19 + 81) as mobile phase and diode array detection. Sample preparation includes deproteinization with papain and purification in a polyamide column. The relative standard deviation for repeated determinations of carminic acid in a commercial strawberry-flavored yogurt was 3.0%. Recoveries of carminic acid added to a natural-flavored yogurt ranged from 87.2 to 95.3% with a mean of 90.2%. The method permits measurement of amounts as low as 0.10 mg/kg.
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