These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Effect of amn gene deletion on Corynebacterium glutamicum S9114 metabolism].
    Author: Mei J, Liu L, Wu J.
    Journal: Wei Sheng Wu Xue Bao; 2015 Dec 04; 55(12):1568-75. PubMed ID: 27101699.
    Abstract:
    OBJECTIVE: To study the effect of adenosine monophosphate nucleosidase gene deletion on Corynebacterium glutamicum S9114 metabolism. METHODS: We constructed the amn deficient strain A amn and studied its glutamate fermentation and acid tolerance. RESULTS: Compared with the wild type strain, biomass of A amn strain increased by 16.2% whereas glutamate production reduced by 58.8%. After 10, 25 and 40 h, intracellular ATP level of A amn strain increased 3.0, 3.7 and 2.2 times. The isocitrate lyase activity increased by 17.1% , 4.9% and 44.5%, the isocitrate dehydrogenase activity decreased by 76.9%, 74.6% and 5.0%, and the glutamate dehydrogenase activity decreased by 42.4%, 50.8% and 42.4%. At pH4.0, the survival of Aamn strain dropped 64.9%. However, the intracellular ROS and protein carbonylation level showed an increase of 31.5% and 22.5% respectively. CONCLUSION: Knocking out of amn gene enhanced intracellular ATP level and biomass formation, whereas had negative effect on glutamate fermentation and acid tolerance.
    [Abstract] [Full Text] [Related] [New Search]