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  • Title: Spatial-temporal analysis of polyethylene glycol-reduced aluminium accumulation and xyloglucan endotransglucosylase action in root tips of common bean (Phaseolus vulgaris).
    Author: Zhang M, Ma Y, Horst WJ, Yang ZB.
    Journal: Ann Bot; 2016 Jul; 118(1):1-9. PubMed ID: 27106549.
    Abstract:
    BACKGROUND AND AIMS: Aluminium (Al) toxicity and drought are two major limiting factors for common bean (Phaseolus vulgaris) production on tropical acid soils. Polyethylene glycol (PEG 6000)-induced osmotic stress (OS) simulating drought stress reduces Al accumulation in the entire root tips of common bean by alteration of cell-wall (CW) porosity, which might be regulated by two genes encoding xyloglucan endotransglucosylase/hydrolase, PvXTH9 and PvXTHb The aim of this research was to understand the spatial and temporal regulation of both XTH genes in PEG-mediated Al accumulation in the root tips. METHODS: In this study the spatial and temporal expression patterns of Al-inhibited root elongation, Al accumulation, XTH gene expression and xyloglucan endotransglucosylase (XET) enzyme action in the root tips were analysed under PEG-induced OS by a combination of physiological and molecular approaches such as quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and in situ fluorescence detection of XET in root tips. KEY RESULTS: The results showed that Al accumulation, expression of XTH genes and XET action were distinctly reduced in the apical 0-2, 2-7 and 7-12 mm zones under OS, implying a potential regulatory role of XTH genes and XET enzyme in CW Al accumulation in these zones. CONCLUSIONS: The results provide novel insights into the physiological and molecular mechanisms of CW structure modification as a response of plant roots to OS, which will contribute to mitigate Al and drought stresses, severely limiting crop yields on acid soils.
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