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  • Title: A CRISPR/Cas9 system adapted for gene editing in marine algae.
    Author: Nymark M, Sharma AK, Sparstad T, Bones AM, Winge P.
    Journal: Sci Rep; 2016 Apr 25; 6():24951. PubMed ID: 27108533.
    Abstract:
    Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.
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