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  • Title: Targeted silencing of Survivin in cancer cells by siRNA loaded chitosan magnetic nanoparticles.
    Author: Unsoy G, Gunduz U.
    Journal: Expert Rev Anticancer Ther; 2016 Jul; 16(7):789-97. PubMed ID: 27130312.
    Abstract:
    BACKGROUND: The aim of this study was to silence Survivin expression, related with drug-resistance, via siRNA-loaded CS-MNPs. METHODS AND RESULTS: The highest siRNA-loading efficiency was achieved at siRNA:CS-MNP ratio of 1:2. Nanoparticles had spherical morphology and homogenous size distribution in TEM. After siRNA loading, core sizes (3-5 nm) of CS-MNPs didn't change significantly, however hydrodynamic diameters increased ~10 nm, indicating swelling of chitosan coat due to efficient siRNA loading. 73% of siRNA was pH-dependently released at 24hours, after 30% burst release at first 3.5hours. Stability was high enough to keep siRNAs in CS-MNPs at pH7.2. Cellular-internalization of Survivin-siRNA-CS-MNPs was high and localized in cytoplasm of cells. CONCLUSION: Although, mock-siRNA loaded/unloaded CS-MNPs weren't cytotoxic, cell-death of breast cancer cells was significantly increased, after the treatment of Survivin-siRNA-loaded CS-MNPs. This reveals, successful loading of Survivin-siRNA on CS-MNPs and significant silencing of Survivin expression by triggering cell-death. Consequently, CS-MNPs are highly efficient delivery systems for intact siRNAs.
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