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  • Title: Plasmid DNA delivery by arginine-rich cell-penetrating peptides containing unnatural amino acids.
    Author: Kato T, Yamashita H, Misawa T, Nishida K, Kurihara M, Tanaka M, Demizu Y, Oba M.
    Journal: Bioorg Med Chem; 2016 Jun 15; 24(12):2681-7. PubMed ID: 27132868.
    Abstract:
    Cell-penetrating peptides (CPPs) have been developed as drug, protein, and gene delivery tools. In the present study, arginine (Arg)-rich CPPs containing unnatural amino acids were designed to deliver plasmid DNA (pDNA). The transfection ability of one of the Arg-rich CPPs examined here was more effective than that of the Arg nonapeptide, which is the most frequently used CPP. The transfection efficiencies of Arg-rich CPPs increased with longer post-incubation times and were significantly higher at 48-h and 72-h post-incubation than that of the commercially available transfection reagent TurboFect. These Arg-rich CPPs were complexed with pDNA for a long time in cells and effectively escaped from the late endosomes/lysosomes into the cytoplasm. These results will be helpful for designing novel CPPs for pDNA delivery.
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