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  • Title: Identification of the heat-labile hemolysin of Actinobacillus pleuropneumoniae serotype 1.
    Author: Devenish J, Rosendal S.
    Journal: Can J Vet Res; 1989 Apr; 53(2):251-4. PubMed ID: 2713790.
    Abstract:
    The heat-labile hemolysin of Actinobacillus pleuropneumoniae serotype 1 strain CM-5 was partially purified using ammonium sulfate precipitation and gel permeation chromatography. This partially purified material was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto nylon filters. The filters were treated with convalescent pig serum and subsequently with CM-5 culture supernatant containing active hemolysin. A 104 kd peptide was identified as the hemolysin because it bound antibodies in convalescent pig serum which cross-linked active hemolysin. The same 104 kd protein when injected into a rabbit produced neutralizing antibodies to the CM-5 hemolysin in culture supernatant.
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