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  • Title: In Vivo Analysis of Intestinal Mononuclear Phagocytes.
    Author: Curato C, Bernshtein B, Aychek T, Jung S.
    Journal: Methods Mol Biol; 2016; 1423():255-68. PubMed ID: 27142022.
    Abstract:
    The study of the intestinal dendritic cell (DC) compartment, its homeostasis, regulation, and response to challenges calls for the investigation within the physiological tissue context comprising the unique anatomic constellation of the epithelial single cell layer and the luminal microbiota, as well as neighboring immune and nonimmune cells. Here we provide protocols we developed that use a combination of conditional cell ablation, conditional compartment mutagenesis, and adoptive precursor transfers to study DC and other intestinal mononuclear phagocytes in in vivo context. We will highlight pitfalls and strengths of these approaches.
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