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Title: Marine pollution risk in a coastal city: use of an eco-genotoxic tool as a stress indicator in mussels from the Eastern Aegean Sea. Author: Kacar A, Pazi I, Gonul T, Kucuksezgin F. Journal: Environ Sci Pollut Res Int; 2016 Aug; 23(16):16067-78. PubMed ID: 27146544. Abstract: Coastal areas, such as bays, estuaries, and harbors, are heavily polluted since these areas are the settlements to which toxic chemicals from industrial and domestic wastes are discharged. The genetic damage was evaluated using bioindicator mussel Mytilus galloprovincialis caused by toxic chemicals (metals and polycyclic aromatic hydrocarbons) in İzmir and Çandarlı Bays (the Eastern Aegean Sea) through comet assay. Three sampling sites from the two bays were selected and the study was conducted during the spring and autumn periods. The highest levels of DNA damage expressed as %Tail-DNA were observed in İzmir Bay (34.60 % Tail-DNA) in the spring. Analysis of the correlation between PAHs and metals in mussels and %T-DNA in the hemolymph and gill cells showed a statistically significant positive correlation between %T-DNA and ∑PAH, chromium (p < 0.05). This study determined the pollution level of the İzmir and Çandarlı Bays by using the DNA damage to the mussel, which can identify the effects of environmental pollutants at the cellular levels. These results confirm that comet assay can be used to determine the temporal and spatial differences of DNA damage, and as a suitable tool for the measurement of genotoxicity in regions with low pollutant concentrations.[Abstract] [Full Text] [Related] [New Search]