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  • Title: Kinetic characterization of GES-22 β-lactamase harboring the M169L clinical mutation.
    Author: Saral A, Leonard DA, Duzgun AO, Cicek AC, June CM, Sandalli C.
    Journal: J Antibiot (Tokyo); 2016 Dec; 69(12):858-862. PubMed ID: 27168312.
    Abstract:
    The class A β-lactamase GES-22 has been identified in Acinetobacter baumannii isolates in Turkey, and subsequently shown to differ from GES-11 by a single substitution (M169L). Because M169 is part of the omega loop, a structure that is known to have major effects on substrate selectivity in class A β-lactamases, we expressed, purified and kinetically characterized this novel variant. Our results show that compared with GES-116 × His, GES-226 × His displays more efficient hydrolysis of penicillins, and aztreonam, but a loss of efficiency against ceftazidime. In addition, the M169L substitution confers on GES-22 more efficient hydrolysis of the mechanistic inhibitors clavulanic acid and sulbactam. These effects are highly similar to other mutations at the homologous position in other class A β-lactamases, suggesting that this methionine has a key structural role in aligning active site residues and in substrate selectivity across the class.
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