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  • Title: [Use of a gelatin substrate for culturing diploid human skin fibroblasts].
    Author: Nizheradze KA, Evdokimova NIu.
    Journal: Tsitologiia; 1989 Jan; 31(1):114-6. PubMed ID: 2718257.
    Abstract:
    Gelatin coating of the growth surface of commercially available polystyrene Petri dishes is proposed for successful cultivation of human skin fibroblasts. A comparison of culture growth dynamics on gelatin, glass and polystyrene allowed to recommend gelatin as the substrate in order to receive an abundant similar material with a low population density viability. The absence of changes in carbohydrate metabolism changes during cell culturing on gelatin provides an opportunity to use these cells in studying regulation of carbohydrate metabolism.
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