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  • Title: Discriminatory usefulness of pulsed-field gel electrophoresis and sequence-based typing in Legionella outbreaks.
    Author: Quero S, García-Núñez M, Párraga-Niño N, Barrabeig I, Pedro-Botet ML, de Simon M, Sopena N, Sabrià M.
    Journal: Future Microbiol; 2016 Jun; 11():757-65. PubMed ID: 27191711.
    Abstract:
    AIM: To compare the discriminatory power of pulsed-field gel electrophoresis (PFGE) and sequence-based typing (SBT) in Legionella outbreaks for determining the infection source. MATERIALS & METHODS: Twenty-five investigations of Legionnaires' disease were analyzed by PFGE, SBT and Dresden monoclonal antibody. RESULTS: The results suggested that monoclonal antibody could reduce the number of Legionella isolates to be characterized by molecular methods. The epidemiological concordance PFGE-SBT was 100%, while the molecular concordance was 64%. Adjusted Wallace index (AW) showed that PFGE has better discriminatory power than SBT (AWSBT→PFGE = 0.767; AWPFGE→SBT = 1). The discrepancies appeared mostly in sequence type (ST) 1, a worldwide distributed ST for which PFGE discriminated different profiles. CONCLUSION: SBT discriminatory power was not sufficient verifying the infection source, especially in worldwide distributed STs, which were classified into different PFGE patterns.
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