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  • Title: Determination of ibuprofen enantiomers in human plasma by HPLC-MS/MS: validation and application in neonates.
    Author: Chen T, Li Q, Lu J, Yu C, Chen C, Li Z.
    Journal: Bioanalysis; 2016 Jun; 8(12):1237-50. PubMed ID: 27225837.
    Abstract:
    AIM: An adaptive method to determine ibuprofen enantiomers with limited volume of plasma required is necessary for investigating PK of ibuprofen in neonates. RESULTS: Enantiomer separation was achieved on a Lux cellulose 3 column with mobile phase consisting of methanol water (85:15, v/v) and formic acid (0.0075%) at isocratic rate of 0.2 ml/min. Calibration curve is linear for each enantiomer at the range of 0.1-60 μg/ml. Validation was conducted and results met requirements regarding to intra- and inter-run precision, accuracy and recovery. No matrix effect or interference was observed from neonatal plasma or comedications. Only 20 μl of plasma was requested in this study. CONCLUSION: This assay was specific and reliable to quantify ibuprofen enantiomers in neonate plasma.
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