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  • Title: Bovine fetal infection with Mycobacterium paratuberculosis.
    Author: Seitz SE, Heider LE, Heuston WD, Bech-Nielsen S, Rings DM, Spangler L.
    Journal: J Am Vet Med Assoc; 1989 May 15; 194(10):1423-6. PubMed ID: 2722634.
    Abstract:
    In utero transmission of Mycobacterium paratuberculosis, the causative agent of paratuberculosis in cattle, has been suggested. Tissue specimens were obtained at a packing plant from pregnant dairy cows and their fetuses and from cows with clinical signs of paratuberculosis and from their fetuses. Specimens were processed according to methods described for isolating M paratuberculosis from bovine tissues and were incubated on Herrold egg yolk medium for 16 weeks. Presumed positive specimens were confirmed to be M paratuberculosis, using acid-fast staining and subculturing. Of 407 lymph nodes from cows, 34 (8.4%) were culture positive for M paratuberculosis; 9 of 34 (26.4%) of these culture-positive cows had fetuses from which specimens were also culture positive. The results estimated the risk of fetal infection with M paratuberculosis to be 26.4% (95% confidence interval between 11.3 and 40.7%).
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