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  • Title: Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage.
    Author: Boros G, Karikó K, Muramatsu H, Miko E, Emri E, Hegedűs C, Emri G, Remenyik É.
    Journal: Methods Mol Biol; 2016; 1428():219-28. PubMed ID: 27236802.
    Abstract:
    In vitro-synthesized mRNA containing nucleoside modifications has great therapeutical potential to transiently express proteins with physiological importance. One such protein is photolyase which rapidly removes UV-induced DNA damages, but this enzyme is absent in humans. Here, we apply a novel mRNA-based platform to achieve functional nonhuman photolyase production in cultured human keratinocytes. Transfection of nucleoside-modified mRNA encoding photolyase leads to accelerated repair of DNA photolesions in human keratinocytes.
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